产品货号：
GS2106
中文名称：
PspN4I内切酶
英文名称：
PspN4I
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：PspN4I内切酶，PspN4I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GGN↑NCC |
| 来源 | Pseudomonas species N4 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme more than 950%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 30 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by 5`-GGNN(5mC)C-3`/3-C(5mC)NNGG-5` or 5`-GGNN(5mC)C-3`/3-CCNNGG-5` methylation |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BmiI,BscBI,BspLI,NlaIV |
相关搜索：PspN4I内切酶，PspN4I限制性内切酶